研究成果Achievements

2021年

     *equal contribution  #corresponding author
 

  1. Ribosomal stress induces 2-cell embryo-like state transition of the mouse ESCs through p53 activation.
    Takahiro M, Kimura Y, Nakano T, #Yamaguchi S.
    Biochem Biophys Res Commun. 2021 Nov 19;579:175-180. doi: 10.1016/j.bbrc.2021.09.068. Epub 2021 Sep 27.

  2. Cell division- and DNA replication-free reprogramming of somatic nuclei for embryonic transcription
    Tomikawa J, Penfold CA , Kamiya T, Hibino R, Kosaka A , Anzai M, Matsumoto K, #Miyamoto K.
    iScience. 2021 Nov 3;24(11):103290. doi: 10.1016/j.isci.2021.103290. eCollection 2021 Nov 19.

  3. The conserved fertility factor SPACA4/Bouncer has divergent modes of action in vertebrate fertilization.
    *Fujihara Y, *Herberg S, Blaha A, Panser K, Kobayashi K, Larasati T, Novatchkova M, Theussl HC, Olszanska O, #Ikawa M, #Pauli A.
    Proc Natl Acad Sci U S A. 2021 Sep 28;118(39):e2108777118. doi: 10.1073/pnas.2108777118.
    紹介記事

  4. Formation of spermatogonia and fertile oocytes in golden hamsters requires piRNAs.
    Loubalova Z, Fulka H, Horvat F, Pasulka J, Malik R, Hirose M, #Ogura A & #Svoboda P.
    Nat Cell Biol. 2021 Sep;23(9):992-1001. doi: 10.1038/s41556-021-00746-2. Epub 2021 Sep 6.
    紹介記事

  5. Production of functional oocytes requires maternally expressed PIWI genes and piRNAs in golden hamsters.
    Hasuwa H, Iwasaki YW, Au Yeung W.K., Ishino K, Masuda H, Sasaki H & #Siomi H.
    Nat Cell Biol. 2021 Sep;23(9):1002-1012. doi: 10.1038/s41556-021-00745-3. Epub 2021 Sep 6.
    紹介記事

  6. SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice.
    Miyata H,  Oura S,  Morohoshi A,  Shimada K, Mashiko D, Oyama Y, Kaneda Y, Matsumura T, Abbasi F, #Ikawa M.
    PNAS. August 31, 2021 118 (35) e2106673118; https://doi.org/10.1073/pnas.2106673118
    紹介記事

  7. RanGTP and the actin cytoskeleton keep paternal and maternal chromosomes apart during fertilization.
    #Mori M, Yao T, Mishina T, Endoh H, Tanaka M, Yonezawa N, Shimamoto Y, Yonemura S, Yamagata K, Kitajima TS, #Ikawa M.
    J Cell Biol. 2021 Oct 4;220(10):e202012001. doi: 10.1083/jcb.202012001. Epub 2021 Aug 23.
    紹介記事

  8. Cdc42 is required for male germline niche development in mice.
    Mori Y, Takashima S, Kanatsu-Shinohara M, Yi Z, #Shinohara T.
    Cell Rep. Volume 36, Issue 7, 17 August 2021, 109550.
    紹介記事

  9. Improved development of mouse somatic cell nuclear transfer embryos by chlamydocin analogues, class I and IIa histone deacetylase inhibitors†.
    Kamimura S, Inoue K, Mizutani E, Kim JM, Inoue H, Ogonuki N, Miyamoto K, Ihashi S, Itami N, Wakayama T, Ito A, Nishino N, Yoshida M, #Ogura A.
    Biol Reprod. 2021 Aug 3;105(2):543-553. doi: 10.1093/biolre/ioab096.

  10. Piwi-piRNA complexes induce stepwise changes in nuclear architecture at target loci.
    #Iwasaki YW, *Sriswasdi S, *Kinugasa Y, Adachi J, Horikoshi Y, Shibuya A, Iwasaki W, Tashiro S, Tomonaga T, #Siomi H.
    EMBO J. 2021 Sep 15;40(18):e108345. doi: 10.15252/embj.2021108345. Epub 2021 Aug 2.
    紹介記事

  11. CRISPR/Cas9-mediated genome editing reveals 12 testis-enriched genes dispensable for male fertility in mice.
    Oyama Y, #Miyata H, Shimada K, Fujihara Y, Tokuhiro K, Garcia TX, Matzuk MM, #Ikawa M.
    Asian J Androl. 2021 Jul 16. doi: 10.4103/aja.aja_63_21. Online ahead of print.

  12. Tet1 regulates epigenetic remodeling of the pericentromeric heterochromatin and chromocenter organization in DNA hypomethylated cells.
    Hagihara Y, Asada S, Maeda T, Nakano T, #Yamaguchi S.
    PLoS Genet. 2021 Jun 24;17(6):e1009646. doi: 10.1371/journal.pgen.1009646. eCollection 2021 Jun.

  13. Spermatogonial stem cell transplantation into nonablated mouse recipient testes.
    Morimoto H, Ogonuki N, Kanatsu-Shinohara M, Matoba S, Ogura A, #Shinohara T.
    Stem Cell Reports. 2021 Jul 13;16(7):1832-1844. doi: 10.1016/j.stemcr.2021.05.013. Epub 2021 Jun 17.

  14. Transcriptome analysis of gene expression changes upon enzymatic dissociation in skeletal myoblasts.
    Miyawaki-Kuwakado A, Wu Q, Harada A, Tomimatsu K, Fujii T, Maehara K, #Ohkawa Y.
    Genes Cells. 2021 Jul;26(7):530-540. doi: 10.1111/gtc.12870. Epub 2021 Jun 8.

  15. Precise CAG repeat contraction in a Huntington's Disease mouse model is enabled by gene editing with SpCas9-NG.
    Oura S, Noda T, Morimura N, Hitoshi S, Nishimasu H, Nagai Y, Nureki O, #Ikawa M.
    Commun Biol. 2021 Jun 23;4(1):771. doi: 10.1038/s42003-021-02304-w.

  16. Capturing human trophoblast development with naive pluripotent stem cells in vitro.
    Io S, Kabata M, Iemura Y, Semi K, Morone N, Minagawa A, Wang B, Okamoto I, Nakamura T, Kojima Y, Iwatani C, Tsuchiya H, Kaswandy B, Kondoh E, Kaneko S, Woltjen K, Saitou M, Yamamoto T, Mandai M, #Takashima Y.
    Cell Stem Cell. 2021 Jun 3;28(6):1023-1039.e13. doi: 10.1016/j.stem.2021.03.013. Epub 2021 Apr 7.

  17. KCTD19 and its associated protein ZFP541 are independently essential for meiosis in male mice.
    Oura S, Koyano T, Kodera C, Horisawa-Takada Y, Matsuyama M, Ishiguro KI, #Ikawa M.
    PLoS Genet. 2021 May 7;17(5):e1009412. doi: 10.1371/journal.pgen.1009412. eCollection 2021 May.

  18. Symmetrically dimethylated histone H3R2 promotes global transcription during minor zygotic genome activation in mouse pronuclei.
    #Morita K, Hatanaka Y, Ihashi S, Asano M, Miyamoto K, Matsumoto K.
    Sci Rep. 2021 May 12;11(1):10146. doi: 10.1038/s41598-021-89334-w.

  19. Maintenance DNA methylation in pre-meiotic germ cells regulates meiotic prophase by facilitating homologous chromosome pairing .
    Takada Y, Yaman-Deveci R, Shirakawa T, Sharif J, Tomizawa SI, Miura F, Ito T, Ono M, Nakajima K, Koseki Y, Shiotani F, Ishiguro KI, #Ohbo K, #Koseki H.
    Development. 2021 May 15;148(10):dev194605. doi: 10.1242/dev.194605. Epub 2021 May 17.

  20. Guiding functions of the C-terminal domain of topoisomerase IIα advance mitotic chromosome assembly.
    Keishi Shintomi & #Tatsuya Hirano
    Nat Commun. 2021 May 18;12(1):2917. doi: 10.1038/s41467-021-23205-w.
    紹介記事

  21. Establishment of novel common marmoset embryonic stem cell lines under various conditions.
    Kishimoto K, Shimada A, Shinohara H, Takahashi T, Yamada Y, Higuchi Y, Yoneda N, Suemizu H, Kawai K, Kurotaki Y, Hanazawa K, Takashima Y, #Sasaki E.
    Stem Cell Res. 2021 May;53:102252. doi: 10.1016/j.scr.2021.102252. Epub 2021 Feb 25.

  22. Structural alteration of the nucleus for the reprogramming of gene expression.
    Tomikawa J, #Miyamoto K.
    FEBS J. 2021 Apr 23. doi: 10.1111/febs.15894. Online ahead of print.

  23. Lipid droplets are formed in 2-cell-like cells. 
    Furuta A, #Nakamura T.
    J Reprod Dev. 2021 Apr 21;67(2):79-81. doi: 10.1262/jrd.2021-003. Epub 2021 Feb 10.

  24. Tsga8 is required for spermatid morphogenesis and male fertility in mice.
    Kobayashi Y, Tomizawa SI, Ono M, Kuroha K, Minamizawa K, Natsume K, Dizdarević S, Dočkal I, Tanaka H, Kawagoe T, Seki M, Suzuki Y, Ogonuki N, Inoue K, Matoba S, Anastassiadis K, Mizuki N, Ogura A, #Ohbo K.
    Development. 2021 Apr 15;148(8):dev196212. doi: 10.1242/dev.196212. Epub 2021 Apr 26.

  25. Dynamics of the cell fate specifications during female gametophyte development in Arabidopsis.
    Susaki D, Suzuki T, Maruyama D, Ueda M, #Higashiyama T, #Kurihara D.
    PLoS Biol. 2021 Mar 26;19(3):e3001123. doi: 10.1371/journal.pbio.3001123. eCollection 2021 Mar.
    紹介記事

  26. Visualization of endogenous nuclear F-actin in mouse embryos reveals abnormal actin assembly after somatic cell nuclear transfer.
    Shindo T, Ihashi S, Sakamoto Y, Okuno T, Tomikawa J, #Miyamoto K.
    J Biochem. 2021 Apr 18;169(3):303-311. 

  27. H2AK119ub1 guides maternal inheritance and zygotic deposition of H3K27me3 in mouse embryos.
    Mei H, Kozuka C, Hayashi R, Kumon M, Koseki H, #Inoue A.
    Nat Genet. 2021 Apr;53(4):539-550. 
    紹介記事

  28. Dynamic regulation of anterior-posterior patterning genes in living Drosophila embryos.
    #Fukaya T.
    Current Biology. Available online 23 March 2021. doi.org/10.1016/j.cub.2021.02.050
    紹介記事

  29. Hamster PIWI proteins bind to piRNAs with stage-specific size variations during oocyte maturation.
    Ishino K, Hasuwa H, Yoshimura J, Iwasaki YW, Nishihara H, Seki NM, Hirano T, Tsuchiya M, Ishizaki H, Masuda H, Kuramoto T, Saito K, Sakakibara Y, Toyoda A, Itoh T, Siomi MC, Morishita S, #Siomi H.
    Nucleic Acids Res. 2021 Mar 18;49(5):2700-2720. doi: 10.1093/nar/gkab059.
    紹介記事

  30. OGG1 protects mouse spermatogonial stem cells from reactive oxygen species in culture†.
    Mori Y, Ogonuki N, Hasegawa A, Kanatsu-Shinohara M, Ogura A, Wang Y, McCarrey JR, #Shinohara T.
    Biol Reprod. 2021 Mar 11;104(3):706-716.

  31. Highly successful production of viable mice derived from vitrified germinal vesicle oocytes.
    Kamoshita M, Fujiwara K, Ito J, Kashiwazaki N.
    PLoS One. 2021 Mar 11;16(3):e0248050. doi: 10.1371/journal.pone.0248050. eCollection 2021.

  32. ClearSeeAlpha: Advanced Optical Clearing for Whole-Plant Imaging.
    #Kurihara D, Mizuta Y, Nagahara S, Higashiyama T.
    Plant Cell Physiol. 2021 Nov 10;62(8):1302-1310. doi: 10.1093/pcp/pcab033.

  33. ARMC12 regulates spatiotemporal mitochondrial dynamics during spermiogenesis and is required for male fertility.
    Shimada K, Park S, Miyata H, Yu Z, Morohoshi A, Oura S, #Matzuk MM, #Ikawa M.
    Proc Natl Acad Sci U S A. 2021 Feb 9;118(6):e2018355118.
    紹介記事

  34. Reprogramming of the histone H3.3 landscape in the early mouse embryo.
    #Ishiuchi T, Abe S, Inoue K, Yeung WKA, Miki Y, Ogura A, #Sasaki H.
    Nat Struct Mol Biol. 2021 Jan;28(1):38-49.

  35. An interplay of NOX1-derived ROS and oxygen determines the spermatogonial stem cell self-renewal efficiency under hypoxia.
    Morimoto H, Yamamoto T, Miyazaki T, Ogonuki N, Ogura A, Tanaka T, Kanatsu-Shinohara M, Yabe-Nishimura C, Zhang H, Pommier Y, Trumpp A, #Shinohara T.
    Genes Dev. 2021 Feb 1;35(3-4):250-260. doi: 10.1101/gad.339903.120. Epub 2021 Jan 14.
    紹介記事  

  36. Genome-wide analysis of chromatin structure changes upon MyoD binding in proliferative myoblasts during the cell cycle.
    Wu Q, Fujii T, Harada A, Tomimatsu K, Miyawaki-Kuwakado A, Fujita M, Maehara K, #Ohkawa Y.
    J Biochem. 2021 Sep 7;169(6):653-661. doi: 10.1093/jb/mvab001.

  37. Protocol for isolation of spermatids from mouse testes.
    #Kim CR, Noda T, Okada Y, #Ikawa M, #Baek SH.
    STAR Protoc. 2021 Jan 8;2(1):100254. 

  38. Generation of chimeric mice with spermatozoa fully derived from embryonic stem cells using a triple-target CRISPR method for Nanos3.
    Miura K, Matoba S, Hirose M, #Ogura A. #Correspondence
    Biol Reprod. 2021 Jan 4;104(1):223-233. 

  39. Development of assisted reproductive technologies for Mus spretus
    Hasegawa A, #Mochida K, Matoba S, Inoue K, Hama D, Kadota M, Hiraiwa N, Yoshiki A, #Ogura A. #Correspondence
    Biol Reprod. 2021 Jan 4;104(1):234-243. doi: 10.1093/biolre/ioaa177.

*equal contribution  #corresponding author

  1. Nucleoskeleton proteins for nuclear dynamics.
    #Miyamoto K, #Harata M. 
    J Biochem. 2021 Apr 18;169(3):237-241. doi: 10.1093/jb/mvab006.
  2. Structural alteration of the nucleus for the reprogramming of gene expression. 
    Tomikawa J, #Miyamoto K. 
    FEBS J. 2021 Apr 23. doi: 10.1111/febs.15894. Epub ahead of print. 
  3. 25th ANNIVERSARY OF CLONING BY SOMATIC-CELL NUCLEAR TRANSFER: Epigenetic abnormalities associated with somatic cell nuclear transfer. 
    #Ogura A, Matoba S, Inoue K. 
    Reproduction. 2021 Jun 11;162(1):F45-F58. doi: 10.1530/REP-21-0013. 
  4. 「世代を超えるエピゲノム」
    (企画:井上、執筆:井上、岡江、小倉)
    実験医学 2021年4月号 Vol.39 No.6

 

  1. Maternal epigenetic inheritance by Polycomb repressive complexes
    Azusa Inoue
    EMBO meeting CellBio Virtual 2021, Online, Dec. 2021
  2. Mammalian sperm-oocyte fusion, ever more puzzling
    Masahito Ikawa
    Gametes Meet in the time of COVID-19, Online, Jul. 2021
  3. Maternal epigenetic inheritance by Polycomb repressive complexes
    Azusa Inoue
    KALAS International Symposium 2021, Online, Jul. 2021
  4. Genome Editing and its Application in the Study or Fertilization
    Masahito Ikawa
    American Society of Andrology annual meeting, Online, Apr. 2021
  5. Efficient Generation of KO/KI Rats Using IVF Embryo
    Arata Honda
    WCC2021-World Congress of Cardiology, Online, Mar. 2021
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    新冨圭史
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  6. Maternal epigenetic inheritance by polycomb repressive complexes
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    宮本 圭 2021.01.12
  2. 学会賞(基礎部門)(日本アンドロロジー学会 第39回学術大会)
    諸星 茜 2021.01.16
  3. 論文賞(日本応用動物昆虫学会)
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