研究成果Achievements

2020年

  1. Exploring trophoblast-specific Tead4 enhancers through chromatin conformation capture assays followed by functional screening.
    Tomikawa J*, Takada S, Okamura K, Terao M, Ogata-Kawata H, Akutsu H, Tanaka S, Hata K, *Nakabayashi K.
    Nucleic Acids Res. 2020 Jan 10;48(1):278-289.

  2. Epitranscriptomic profiling in human placenta: N6-methyladenosine modification at the 5'-untranslated region is related to fetal growth and preeclampsia
    Taniguchi K, Kawai T*, Kitawaki J, Tomikawa J, Nakabayashi K, Okamura K, Sago H, *Hata K.
    FASEB J. 2020 Jan;34(1):494-512. 

  3. Genetic mutation of Frem3 does not cause Fraser syndrome in mice.
    Kiyozumi D, Mori M, Kodani M, *Ikawa M.
    Exp Anim. 2020 Jan 29;69(1):104-109.

  4. Mouse T-complex protein 11 is important for progressive motility in sperm.
    *Castaneda JM, Miyata H, Archambeault DR, Satouh Y, Yu Z, Ikawa M, Matzuk MM.
    Biol Reprod. 2020;102(4):852–862.

  5. Nexin-Dynein Regulatory Complex component DRC7 but not FBXL13 is required for sperm flagellum formation and male fertility in mice.
    Morohoshi A, Miyata H, Shimada K, Nozawa K, Matsumura T, Yanase R, Shiba K, Inaba K, *Ikawa M.
    PLoS Genet. 2020 Jan 21;16(1):e1008585. 

  6. Acrosin is essential for sperm penetration through the zona pellucida in hamsters.
    Hirose M, Honda A, Fulka H, Tamura-Nakano M, Matoba S, Tomishima T, Mochida K, Hasegawa A, Nagashima K, Inoue K, Ohtsuka M, Baba T, Yanagimachi R, Ogura A.
    Proc Natl Acad Sci USA. 2020;117 (5) 2513-2518.
    紹介記事

  7. PITHD1 is a proteasome-interacting protein essential for male fertilization.
    §Kondo H, §Matsumura T, Kaneko M, Inoue K, Hidetaka K, Ikawa M, Takahama Y, *Ohigashi I.  §:equal contribution
    J Biol Chem. 2020 Feb 7; 295 (6) : 1658-1672.

  8. IgSF11 regulates osteoclast differentiation through association with the scaffold protein PSD-95.
    §Kim H, §Takegahara N, Walsh MC, Middleton SA, Yu J, Shirakawa J, Ueda J, Fujihara Y, Ikawa M, Ishii M, Kim J, *Choi Y.  §: equal contribution.
    Bone Res. 2020 Feb 10;8:5.

  9. Generation of a male common marmoset embryonic stem cell line DSY127-BV8VT1 carrying double reporters specific for the germ cell linage using the CRISPR-Cas9 and PiggyBac transposase systems.
    Yoshimatsu S, Sato T, Yamamoto M, Sasaki E, Nakajima M, Nakamura M, Shiozawa S, Noce T, Okano H*.
    Stem Cell Res. 2020 Feb 21;44:101740.

  10. Expression and functional analyses of ephrin type-A receptor 2 in mouse spermatogonial stem cells†.
    Morimoto H, Kanatsu-Shinohara M, Orwig KE, *Shinohara T.
    Biol Reprod. 2020 Feb 12;102(1):220-232. 

  11. Serum anti-ecoverin antibodies is found in elderly patients with retinitis pigmentosa and cancer.
    Sato T, *Nishiguchi KM, Fujita K, Miya F, Inoue T, Sasaki E, Asano T, Tsuda S, Shiga Y, Kunikata H, Nakazawa M, Nakazawa T.
    Acta Ophthalmol. 2020 Feb 11.

  12. Crystal structure of Drosophila Piwi.
    Yamaguchi S, Oe A, Nishida KM, Yamashita K, Kajiya A, Hirano S, Matsumoto N, Dohmae N, Ishitani R, Saito K, Siomi H, *Nishimasu H, *Siomi MC, Nureki O.
    Nat Commun. 2020 Feb 12;11(1):858. 

  13. The testis-specific serine proteases Prss44, Prss46, and Prss54 are dispensable for mouse fertility.
    Holcomb RJ, Oura S, Nozawa K, Kent K, Yu Z, Robertson MJ, Coarfa C, Matzuk MM, Ikawa M, *Garcia TX.
    Biol Reprod. 2020 Feb 12;102(1):84-91.

  14. Transgenesis and Genome Editing of Mouse Spermatogonial Stem Cells by Lentivirus Pseudotyped with Sendai Virus F Protein.
    *Shinohara T, Kanatsu-Shinohara M.  
    Stem Cell Reports. 2020 Mar 10;14(3):447-461.

  15. Novel gastrointestinal disease in common marmosets characterised by duodenal dilation: a clinical and pathological study.
    Mineshige T, *Inoue T, Yasuda M, Yurimoto T, Kawai K, Sasaki E.
    Sci Rep. 2020 Mar 2;10(1):3793.

  16. DGKγ knockout mice show impairments in cerebellar motor coordination, LTD and the dendritic development of Purkinje cells through the activation of PKCγ.
    Tsumagari R, Kakizawa S, Kikunaga S, Fujihara Y, Ueda S, Yamanoue M, Saito N, Ikawa M, *Shirai Y.
    eNeuro.2020 Mar 4;7(2):ENEURO.0319-19.2020.

  17. Human PZP and common marmoset A2ML1 as pregnancy related proteins.
    Kashiwagi H, Ishimoto H, Izumi SI, Seki T, Kinami R, Otomo A, Takahashi K, Kametani F, Hirayama N, Sasaki E, Shiina T, Sakabe K, Mikami M, *Kametani Y.
    Sci Rep. 2020 Mar 20;10(1):5088.

  18. Primed to naïve-like conversion of the common marmoset embryonic stem cells.
    Shiozawa S*, Nakajima M, Okahara J, Kuortaki Y, Kisa F, Yoshimatsu S, Nakamura M, Koya I, Yoshimura M, Sasagawa Y, Nikaido I, Sasaki E, Okano H*.
    Stem Cells Dev. 2020 Mar 18.

  19. The actin-family protein Arp4 is a novel suppressor for the formation and functions of nuclear F-actin.
    Yamazaki S, Gerhold C, Yamamoto K, Ueno Y, Grosse R, Miyamoto K, *Harata M.
    Cells. 2020 Mar 19;9(3):758. 

  20. Structural Insights Into Tetraspanin CD9 Function.
    §Umeda R, §Satouh Y, Takemoto M, Nakada-Nakura Y, Liu K, Yokoyama T, Shirouzu M, Iwata S, Nomura N, Sato K, Ikawa M, *Nishizawa T, *Nureki O.  §:equal contribution.
    Nat Commun. 2020 Mar 30;11(1):1606.

  21. CRISPR/Cas9-mediated genome editing reveals Oosp family genes are dispensable for female fertility in mice.
    §Abbasi F, §Kodani M, Emori C, Kiyozumi D, Mori M, Fujihara Y, *Ikawa M.  §:equal contribution.
    Cells. 2020 Mar 28;9(4):E821.

  22. Autologous transplantation of spermatogonial stem cells restores fertility in congenitally infertile mice.
    Kanatsu-Shinohara M, Ogonuki M, Matoba S, Ogura A, Shinohara T.
    PNAS 2020 Apr 7;117(14):7837-7844.
    紹介記事   

  23. A pilot study to establish human T-cell leukemia virus type 1 (HTLV-1) carrier model using common marmoset (Callithrix jacchus).
    Hirose L, Hiramoto T, Tian Y, Kohara H, Kobayashi S, Nagai E, Denda T, Tanaka Y, Ota Y, Jiyuan L, Miyamoto S, Miura Y, Hijikata Y, Soda Y, Inoue T, Okahara N, Itoh T, Sasaki E, Tojo A, Uchimaru K, *Tani K.
    J Med Primatol. 2020 Apr;49(2):86-94.

  24. Testis-enriched kinesin KIF9 is important for progressive motility in mouse spermatozoa.
    *Miyata H, Shimada K, Morohoshi A, Oura S, Matsumura T, Xu Z, Oyama Y, *Ikawa M.
    FASEB J. 2020 Apr;34(4):5389-5400.

  25. TESMIN, METALLOTHIONEIN-LIKE 5, is required for spermatogenesis.
    Oji A, Isotani A, Fujihara Y, Castaneda JM, Oura S, *Ikawa M.
    Biol Reprod. 2020 Apr 15;102(4):975-983.

  26. Spermatozoa Lacking Fertilization Influencing Membrane Protein (FIMP) Fail to Fuse With Oocytes in Mice.
    Fujihara Y, Lu Y, Noda T, Oji A, Larasati T, Kojima-Kita K, Yu Z, Matzuk RM, *Matzuk MM, *Ikawa M.
    Proc Natl Acad Sci U S A. 2020 Apr 28;117(17):9393-9400. 
    紹介記事   

  27. Prss55 but Not Prss51 Is Required for Male Fertility in Mice.
    Kobayashi K, Endo T, Matsumura T, Lu Y, Yu Z, *Matzuk MM, *Ikawa M.
    Biol Reprod. 2020 Apr 17:ioaa041. Online ahead of print.

  28. Loss of H3K27me3 Imprinting in the Sfmbt2 miRNA Cluster Causes Enlargement of Cloned Mouse Placentas.
    *Inoue K, Ogonuki N, Kamimura S, Inoue H, Matoba S, Hirose M, Honda A, Miura K, Hada M, Hasegawa A, Watanabe N, Dodo Y, Mochida K, *Ogura A.
    Nat Commun. 2020 May 1;11(1):2150.
    紹介記事  

  29. Identification of transmembrane protein 168 mutation in familial Brugada syndrome.
    §Shimizu A, §Zankov DP, Sato A, Komeno M, Toyoda F, Yamazaki S, Makita T, Noda T, Ikawa M, Asano Y, Miyashita Y, Takashima S, Morita H, Ishikawa T, Makita N, Hitosugi M, Matsuura H, Ohno S, Horie M, *Ogita H. §:equal contribution.
    FASEB J. 2020 2020 May;34(5):6399-6417.

  30. Sperm proteins SOF1, TMEM95, and SPACA6 are required for sperm-oocyte fusion in mice.
    Noda T, Lu Y, Fujihara Y, Oura S, Koyano T, Kobayashi S, Matzuk MM,  *Ikawa M.
    Proc Natl Acad Sci U S A. 2020;117(21):11493‐11502.
    紹介記事   

  31. NELL2-mediated lumicrine signaling through OVCH2 is required for male fertility.
    Kiyozumi D, Noda T, Yamaguchi R, Tobita T, Matsumura T, Shimada K, Kodani M, Kohda T, Fujihara Y, Ozawa M, Yu Z, Miklossy G, Bohren MK, Horie M, Okabe M, *Matzuk MM, *Ikawa M.
    Science. 2020:368(6495)1132-1135.
    紹介記事   

  32. Zygotic Nuclear F-Actin Safeguards Embryonic Development.
    Okuno T, Li WY, Hatano Y, Takasu A, Sakamoto Y, Yamamoto M, Ikeda Z, Shindo T, Plessner M, Morita K, Matsumoto K, Yamagata K, Grosse R, *Miyamoto K.
    Cell Rep. 2020 Jun 30;31(13):107824. 

  33. Bi-allelic DNAH8 Variants Lead to Multiple Morphological Abnormalities of the Sperm Flagella and Primary Male Infertility.
    §Liu C, §Miyata H, §Gao Y, §Sha Y, §Tang S, §Xu Z, Whitfield M, Patrat C, Wu H, Dulioust E, Tian S, Shimada K, Cong J, Noda T, Li H, Morohoshi A, Cazin C, Kherraf ZE, Arnoult C, Jin L, He X, Ray PF, Cao Y, Touré A, *Zhang F, *Ikawa M. §:equal contribution.
    AJHG. 2020 Jun 24;S0002-9297(20)30192-0. Online ahead of print.

  34. RNA-binding Protein Ptbp1 Regulates Alternative Splicing and Transcriptome in Spermatogonia and Maintains Spermatogenesis in Concert With Nanos3.
    Senoo M, Hozoji H, Ishikawa-Yamauchi Y, Takijiri T, Ohta S, Ukai T, Kabata M, Yamamoto T, Yamada Y, *Ikawa M, *Ozawa M.
    J Reprod Dev. 2020 Jul 6. Online ahead of print.

  35. Protocadherin-7 Contributes to Maintenance of Bone Homeostasis Through Regulation of Osteoclast Multinucleation.
    Kim H, Takegahara N, Walsh MC, Ueda J, Fujihara Y, Ikawa M, *Choi Y.
    BMB Rep. 2020 Jul 8:4975. Online ahead of print.

  36. Diphtheria toxin-mediated transposon-driven poly (A)-trapping efficiently disrupts transcriptionally silent genes in embryonic stem cells.
    *Bai J, Kondo R, Mayasari NI, Shigeoka T, Isotani A, Ikawa M, Sashida G, Kawaichi M, *Ishida Y.
    Genesis. 2020 Jul 9:e23386. doi: 10.1002/dvg.23386. Online ahead of print.

  1. How to improve mouse cloning.
    Ogura A.
    Theriogenology. 2020 Jan 20;S0093-691X(20)30043-1.
  2. ラミンとクロマチンの連携による核構造の維持メカニズム
    島本勇太, 前島一博
    医学のあゆみ 2020年1月号 Vol. 272 (2): 157-161 医歯薬出版株式会社
  3. 受精卵,ウイルス,ES,筋ジスを巡るDUX発見物語
    井上梓
    実験医学 2020年1月号 Vol.38 No.1, 63-64 (2020)
  1. NELL2 is necessary for epididymal differentiation and male fertility
    淨住大慈
    令和元年 微生物病研究所 研究業績発表会 (大阪府吹田市) 2020.1.17
  2. ピッグと並ぶもう1つの注目動物:マーモセット
    佐々木えりか
    自治医科大学先端医療技術開発センターシンポジウム2020 (栃木県下野市) 2020.2.10
  3. エピゲノム解析が明らかにする卵子・受精卵の特性
    石内崇士
    世界を先導するリプロダクションコアの形成研究会 (岡山県岡山市) 2020.2.27
  4. Intergenerational epigenetic inheritance in mammals
    井上梓
    京都大学iPS研究所(CiRA) retreat 2019 (滋賀県大津市) 2020.2.14
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