研究成果Achievements

2020年

     *equal contribution  #corresponding author

  1. Impairment of nuclear F-actin formation and its relevance to cellular phenotypes in Hutchinson-Gilford progeria syndrome.
    Takahashi Y, Hiratsuka S, Machida N, Takahashi D, Matsushita J, Hozak P, Misteli T, Miyamoto K, #Harata M. #Correspondence
    Nucleus. 2020 Dec;11(1):250-263. 

  2. A histone H3.3K36M mutation in mice causes an imbalance of histone modifications and defects in chondrocyte differentiation.
    Shusaku Abe, Hiroaki Nagatomo, #Hiroyuki Sasaki, #Takashi Ishiuchi. #Correspondence
    Epigenetics. 2020 Nov 16;1-12. doi: 10.1080/15592294.2020.1841873. Online ahead of print.

  3. Expression of Dux family genes in early preimplantation embryos. 
    Sugie K, Funaya S, Kawamura M, Nakamura T, Suzuki MG, #Aoki F. #Correspondence
    Sci Rep. 2020 Nov 10;10(1):19396. 

  4. Reprogramming of the histone H3.3 landscape in the early mouse embryo.
    #Takashi Ishiuchi, Shusaku Abe, Kimiko Inoue, Wan Kin Au Yeung, Yuka Miki, Atsuo Ogura & #Hiroyuki Sasaki. #:Correspondence
    Nature structural & molecular biology. 2020 Nov 09; Online ahead of print.
    紹介記事   

  5. CRISPR/CAS9-mediated amino acid substitution reveals phosphorylation residues of RSPH6A are not essential for male fertility in mice
    #Miyata H, Abbasi F, Visconti PE, #Ikawa M. #Correspondence
    Biol Reprod. 2020 Oct 29;103(5):912-914. 

  6. Chromatin integration labeling for mapping DNA-binding proteins and modifications with low input.
    Handa T, Harada A, Maehara K, Sato S, Nakao M, Goto N, Kurumizaka H, #Ohkawa Y, #Kimura H.
    Nat Protoc. 2020 Oct;15(10):3334-3360. doi: 10.1038/s41596-020-0375-8. Epub 2020 Aug 17.

  7. The mouse Sry locus harbors a cryptic exon that is essential for male sex determination.
    Miyawaki S, Kuroki S, Maeda R, Okashita N, Koopman P, #Tachibana M.
    Science. 2020 Oct 2;370(6512):121-124. doi: 10.1126/science.abb6430.

  8. CD2 is a surface marker for mouse and rat spermatogonial stem cells.
    Kanatsu-Shinohara M, Chen G, Morimoto H, #Shinohara T. #Correspondence
    J Reprod Dev. 2020 Aug 20;66(4):341-349. 

  9. The PRDM14-CtBP1/2-PRC2 complex regulates transcriptional repression during the transition from primed to naïve pluripotency.
    Yamamoto M, Suwa Y, Sugiyama K, Okashita N, Kawaguchi M, Tani N, Matsubara K, Nakamura A, #Seki Y.
    J Cell Sci. 2020 Aug 14;133(15):jcs240176. doi: 10.1242/jcs.240176.

  10. H3K9 Demethylases JMJD1A and JMJD1B Control Prospermatogonia to Spermatogonia Transition in Mouse Germline.
    Kuroki S, Maeda R, Yano M, Kitano S, Miyachi H, Fukuda M, Shinkai Y, #Tachibana M.
    Stem Cell Reports. 2020 Aug 11;15(2):424-438. doi: 10.1016/j.stemcr.2020.06.013. Epub 2020 Jul 16.

  11. Bi-allelic DNAH8 Variants Lead to Multiple Morphological Abnormalities of the Sperm Flagella and Primary Male Infertility.
    *Liu C, *Miyata H, *Gao Y, *Sha Y, *Tang S, *Xu Z, Whitfield M, Patrat C, Wu H, Dulioust E, Tian S, Shimada K, Cong J, Noda T, Li H, Morohoshi A, Cazin C, Kherraf ZE, Arnoult C, Jin L, He X, Ray PF, Cao Y, Touré A, #Zhang F, #Ikawa M. *:equal contribution. #:Correspondence
    Am J Hum Genet. 2020 Aug 6;107(2):330-341. 
    紹介記事   

  12. Knockout of family with sequence similarity 170 member A (Fam170a) causes male subfertility, while Fam170b is dispensable in mice
    Devlin DJ, Nozawa K, Ikawa M, #Matzuk MM. #Correspondence
    Biol Reprod. 2020 Aug 4;103(2):205-222. 

  13. CRISPR/Cas9-mediated genome-edited mice reveal 10 testis-enriched genes are dispensable for male fecundity.
    *Soojin Park, *Keisuke Shimada, Yoshitaka Fujihara, Zoulan Xu, Kentaro Shimada, Tamara Larasati, Putri Pratiwi, Ryan M. Matzuk, Darius J. Devlin, Zhifeng Yu, Thomas X. Garcia, *Martin M. Matzuk, and #Masahito Ikawa. *:equal contribution, #Correspondence
    Biol Reprod. 2020 Aug 4;103(2):195-204.
     
  14. CRISPR/Cas9-based genome editing in mice uncovers 13 testis- or epididymis-enriched genes individually dispensable for male reproduction.
    *Sun J, *Lu Y, Nozawa K, Xu Z, Morohoshi A, Castaneda JM, Noda T, Miyata H, Abbasi F, Shawki HH, Takahashi S, Devlin DJ, Yu Z, Matzuk RM, Garcia TX, #Martin MM, #Ikawa M.*:equal contribution. #:Correspondence
    Biol Reprod . 2020 Aug 4;103(2):183-194.
     
  15. CIB4 is essential for the haploid phase of spermatogenesis in mice.
    *Xu Z, *Miyata H, Kaneda Y, Castaneda JM, Lu Y, Morohoshi A, Yu Z, Matzuk MM, #Ikawa M. *:equal contribution
    Biol Reprod . 2020 Aug 4;103(2):235-243.
     
  16. Tmprss12 is required for sperm motility and uterotubal junction migration in mice.
    *Larasati T, *Noda T, Fujihara Y, Shimada K, Tobita T, Yu Z, #Matzuk MM, #Ikawa M. *:equal contribution
    Biol Reprod . 2020 Aug 4;103(2):254-263.
     
  17. Knockout of serine-rich single-pass membrane protein 1 (Ssmem1) causes globozoospermia and sterility in male mice.
    Nozawa K, Zhang Q, Miyata H, Devlin DJ, Yu Z, Oura S, Koyano T, Matsuyama M, #Ikawa M, #Matzuk MM.
    Biol Reprod . 2020 Aug 4;103(2):244-253.
     
  18. Prss55 but Not Prss51 Is Required for Male Fertility in Mice.
    Kobayashi K, Endo T, Matsumura T, Lu Y, Yu Z, #Matzuk MM, #Ikawa M.
     
  19. PHF7 Modulates BRDT Stability and Histone-to-Protamine Exchange during Spermiogenesis.
    *Kim CR, *Noda T, Kim H, Kim G, Park S, Na Y, Oura S, Shimada K, Bang I, Ahn JY, Kim YR, Oh SK, Choi HJ, Kim JS, Jung I, Lee H, Okada Y, #Ikawa M, #Baek SH. *equal contribution. #Correspondence
    Cell Rep. 2020 Jul 28;32(4):107950. 
     
  20. Zygotic Nuclear F-Actin Safeguards Embryonic Development.
    Okuno T, Li WY, Hatano Y, Takasu A, Sakamoto Y, Yamamoto M, Ikeda Z, Shindo T, Plessner M, Morita K, Matsumoto K, Yamagata K, Grosse R, #Miyamoto K.
    Cell Rep. 2020 Jun 30;31(13):107824. 
    紹介記事   
     
  21. Genome-wide kinetic properties of transcriptional bursting in mouse embryonic stem cells.
    #Ochiai H, Hayashi T, Umeda M, Yoshimura M, Harada A, Shimizu Y, Nakano K, Saitoh N, Liu Z, Yamamoto T, Okamura T, Ohkawa Y, Kimura H, #Nikaido I.
    Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi: 10.1126/sciadv.aaz6699. eCollection 2020 Jun.
     

  22. NELL2-mediated lumicrine signaling through OVCH2 is required for male fertility.
    Kiyozumi D, Noda T, Yamaguchi R, Tobita T, Matsumura T, Shimada K, Kodani M, Kohda T, Fujihara Y, Ozawa M, Yu Z, Miklossy G, Bohren MK, Horie M, Okabe M, #Matzuk MM, #Ikawa M.
    Science. 2020 Jun 5;368(6495):1132-1135. doi: 10.1126/science.aay5134.
    紹介記事   

  23. Sperm proteins SOF1, TMEM95, and SPACA6 are required for sperm-oocyte fusion in mice.
    Noda T, Lu Y, Fujihara Y, Oura S, Koyano T, Kobayashi S, Matzuk MM,  #Ikawa M.
    Proc Natl Acad Sci U S A. 2020 May 26;117(21):11493-11502. doi: 10.1073/pnas.1922650117. Epub 2020 May 11.
    紹介記事   
     
  24. Loss of H3K27me3 Imprinting in the Sfmbt2 miRNA Cluster Causes Enlargement of Cloned Mouse Placentas.
    #Inoue K, Ogonuki N, Kamimura S, Inoue H, Matoba S, Hirose M, Honda A, Miura K, Hada M, Hasegawa A, Watanabe N, Dodo Y, Mochida K, #Ogura A.
    Nat Commun. 2020 May 1;11(1):2150.
    紹介記事  
     

  25. Spermatozoa Lacking Fertilization Influencing Membrane Protein (FIMP) Fail to Fuse With Oocytes in Mice.
    Fujihara Y, Lu Y, Noda T, Oji A, Larasati T, Kojima-Kita K, Yu Z, Matzuk RM, #Matzuk MM, #Ikawa M.
    Proc Natl Acad Sci U S A. 2020 Apr 28;117(17):9393-9400. 
    紹介記事   

  26. Tesmin, Metallothionein-Like 5, is Required for Spermatogenesis in Mice.
    Oji A, Isotani A, Fujihara Y, Castaneda JM, Oura S, #Ikawa M.
    Biol Reprod. 2020 Apr 15;102(4):975-983.
     
  27. Mouse T-complex protein 11 is important for progressive motility in sperm.
    #Castaneda JM, Miyata H, Archambeault DR, Satouh Y, Yu Z, Ikawa M, Matzuk MM.
    Biol Reprod. 2020 Apr 15;102(4):852-862. doi: 10.1093/biolre/ioz226.
     

  28. Testis-enriched kinesin KIF9 is important for progressive motility in mouse spermatozoa.
    #Miyata H, Shimada K, Morohoshi A, Oura S, Matsumura T, Xu Z, Oyama Y, #Ikawa M.
    FASEB J. 2020 Apr;34(4):5389-5400.

  29. A pilot study to establish human T-cell leukemia virus type 1 (HTLV-1) carrier model using common marmoset (Callithrix jacchus).
    Hirose L, Hiramoto T, Tian Y, Kohara H, Kobayashi S, Nagai E, Denda T, Tanaka Y, Ota Y, Jiyuan L, Miyamoto S, Miura Y, Hijikata Y, Soda Y, Inoue T, Okahara N, Itoh T, Sasaki E, Tojo A, Uchimaru K, #Tani K.
    J Med Primatol. 2020 Apr;49(2):86-94.

  30. Autologous transplantation of spermatogonial stem cells restores fertility in congenitally infertile mice.
    Kanatsu-Shinohara M, Ogonuki M, Matoba S, Ogura A, Shinohara T.
    PNAS 2020 Apr 7;117(14):7837-7844.
    紹介記事   

  31. CRISPR/Cas9-mediated genome editing reveals Oosp family genes are dispensable for female fertility in mice.
    *Abbasi F, *Kodani M, Emori C, Kiyozumi D, Mori M, Fujihara Y, #Ikawa M.  *equal contribution.
    Cells. 2020 Mar 28;9(4):E821.

  32. Structural Insights Into Tetraspanin CD9 Function.
    *Umeda R, *Satouh Y, Takemoto M, Nakada-Nakura Y, Liu K, Yokoyama T, Shirouzu M, Iwata S, Nomura N, Sato K, Ikawa M, vNishizawa T, #Nureki O.  *equal contribution.
    Nat Commun. 2020 Mar 30;11(1):1606.

  33. The actin-family protein Arp4 is a novel suppressor for the formation and functions of nuclear F-actin.
    Yamazaki S, Gerhold C, Yamamoto K, Ueno Y, Grosse R, Miyamoto K, #Harata M.
    Cells. 2020 Mar 19;9(3):758. 

  34. Primed to naïve-like conversion of the common marmoset embryonic stem cells.
    #Shiozawa S, Nakajima M, Okahara J, Kuortaki Y, Kisa F, Yoshimatsu S, Nakamura M, Koya I, Yoshimura M, Sasagawa Y, Nikaido I, Sasaki E, #Okano H.
    Stem Cells Dev. 2020 Mar 18.

  35. Human PZP and common marmoset A2ML1 as pregnancy related proteins.
    Kashiwagi H, Ishimoto H, Izumi SI, Seki T, Kinami R, Otomo A, Takahashi K, Kametani F, Hirayama N, Sasaki E, Shiina T, Sakabe K, Mikami M, #Kametani Y.
    Sci Rep. 2020 Mar 20;10(1):5088.

  36. DGKγ knockout mice show impairments in cerebellar motor coordination, LTD and the dendritic development of Purkinje cells through the activation of PKCγ.
    Tsumagari R, Kakizawa S, Kikunaga S, Fujihara Y, Ueda S, Yamanoue M, Saito N, Ikawa M, #Shirai Y.
    eNeuro.2020 Mar 4;7(2):ENEURO.0319-19.2020.

  37. Novel gastrointestinal disease in common marmosets characterised by duodenal dilation: a clinical and pathological study.
    Mineshige T, #Inoue T, Yasuda M, Yurimoto T, Kawai K, Sasaki E.
    Sci Rep. 2020 Mar 2;10(1):3793.

  38. Transgenesis and Genome Editing of Mouse Spermatogonial Stem Cells by Lentivirus Pseudotyped with Sendai Virus F Protein.
    #Shinohara T, Kanatsu-Shinohara M.  
    Stem Cell Reports. 2020 Mar 10;14(3):447-461.

  39. The testis-specific serine proteases Prss44, Prss46, and Prss54 are dispensable for mouse fertility.
    Holcomb RJ, Oura S, Nozawa K, Kent K, Yu Z, Robertson MJ, Coarfa C, Matzuk MM, Ikawa M, #Garcia TX.
    Biol Reprod. 2020 Feb 12;102(1):84-91.

  40. Crystal structure of Drosophila Piwi.
    Yamaguchi S, Oe A, Nishida KM, Yamashita K, Kajiya A, Hirano S, Matsumoto N, Dohmae N, Ishitani R, Saito K, Siomi H, #Nishimasu H, #Siomi MC, Nureki O.
    Nat Commun. 2020 Feb 12;11(1):858. 

  41. Serum anti-recoverin antibodies is found in elderly patients with retinitis pigmentosa and cancer.
    Sato T, #Nishiguchi KM, Fujita K, Miya F, Inoue T, Sasaki E, Asano T, Tsuda S, Shiga Y, Kunikata H, Nakazawa M, Nakazawa T.
    Acta Ophthalmol. 2020 Feb 11.

  42. Expression and functional analyses of ephrin type-A receptor 2 in mouse spermatogonial stem cells†.
    Morimoto H, Kanatsu-Shinohara M, Orwig KE, #Shinohara T.
    Biol Reprod. 2020 Feb 12;102(1):220-232. 

  43. Generation of a male common marmoset embryonic stem cell line DSY127-BV8VT1 carrying double reporters specific for the germ cell linage using the CRISPR-Cas9 and PiggyBac transposase systems.
    Yoshimatsu S, Sato T, Yamamoto M, Sasaki E, Nakajima M, Nakamura M, Shiozawa S, Noce T, #Okano H.
    Stem Cell Res. 2020 Feb 21;44:101740.

  44. IgSF11 regulates osteoclast differentiation through association with the scaffold protein PSD-95.
    *Kim H, *Takegahara N, Walsh MC, Middleton SA, Yu J, Shirakawa J, Ueda J, Fujihara Y, Ikawa M, Ishii M, Kim J, #Choi Y.  *:equal contribution.
    Bone Res. 2020 Feb 10;8:5.

  45. PITHD1 is a proteasome-interacting protein essential for male fertilization.
    *Kondo H, *Matsumura T, Kaneko M, Inoue K, Hidetaka K, Ikawa M, Takahama Y, #Ohigashi I.  *equal contribution
    J Biol Chem. 2020 Feb 7; 295 (6) : 1658-1672.
     

  46. Acrosin is essential for sperm penetration through the zona pellucida in hamsters.
    Hirose M, Honda A, Fulka H, Tamura-Nakano M, Matoba S, Tomishima T, Mochida K, Hasegawa A, Nagashima K, Inoue K, Ohtsuka M, #Baba T, #Yanagimachi R, #Ogura A.
    Proc Natl Acad Sci U S A. 2020 Feb 4;117(5):2513-2518. doi: 10.1073/pnas.1917595117. Epub 2020 Jan 21.
    紹介記事

  47. Nexin-Dynein Regulatory Complex component DRC7 but not FBXL13 is required for sperm flagellum formation and male fertility in mice.
    Morohoshi A, Miyata H, Shimada K, Nozawa K, Matsumura T, Yanase R, Shiba K, Inaba K, #Ikawa M.
    PLoS Genet. 2020 Jan 21;16(1):e1008585. 

  48. Genetic mutation of Frem3 does not cause Fraser syndrome in mice.
    Kiyozumi D, Mori M, Kodani M, #Ikawa M.
    Exp Anim. 2020 Jan 29;69(1):104-109.

  49. Epitranscriptomic profiling in human placenta: N6-methyladenosine modification at the 5'-untranslated region is related to fetal growth and preeclampsia
    Taniguchi K, #Kawai T, Kitawaki J, Tomikawa J, Nakabayashi K, Okamura K, Sago H, #Hata K.
    FASEB J. 2020 Jan;34(1):494-512. 

  50. Exploring trophoblast-specific Tead4 enhancers through chromatin conformation capture assays followed by functional screening.
    #Tomikawa J, Takada S, Okamura K, Terao M, Ogata-Kawata H, Akutsu H, Tanaka S, Hata K, #Nakabayashi K.
    Nucleic Acids Res. 2020 Jan 10;48(1):278-289.

  1. 卵管内の精子輸送メカニズム
    日野 敏昭
    実践 卵管学 2020年12月発行 P87-94 株式会社 中外医学社
  2. 国際宇宙ステーションでのマウス飼育により宇宙滞在が精子受精能力に及ぼす影響を解析
    野田大地，芝大，伊川正人
    Isotope News  2020年8月号 No. 770
  3. 精子が受精能をもつための鍵分子を発見！
    淨住大慈
    化学 2020年8月号（75巻）page 75
  4. CRISPR-Cas9を用いた疾患モデルマウスの作製――不妊モデルマウスを例に
    野田大地，大浦聖矢，伊川正人
    「ゲノム編集の未来」医学のあゆみ 2020年5月30日 医歯薬出版(株) 273(9);P774-779.
  5. 受精卵，ウイルス，ES，筋ジスを巡るDUX発見物語
    井上梓
    実験医学 2020年1月号 Vol.38 No.1, 63-64 (2020)
  6. ラミンとクロマチンの連携による核構造の維持メカニズム
    島本勇太， 前島一博
    医学のあゆみ 2020年1月号 Vol. 272 (2): 157-161 医歯薬出版株式会社
  7. How to improve mouse cloning.
    Ogura A.
    Theriogenology. 2020 Jan 20;S0093-691X(20)30043-1.

  1. Golden hamster as a new model to study the PIWI-piRNA pathway in mammal
    Haruhiko Shiomi
    Cold Spring harbor Laboratory meeting on Regulatory & Non-coding RNAs, Online, May. 2020
  2. Variant PRC1-mediated H2A mono-ubiquitination ensures maternal inheritance of H3K27me3
    Azusa Inoue
    Austrian Acad. Sci. Gregor Mendel Institute of Molecular Plant Biology, Online, May. 2020
  3. Regulation of transcriptional bursting by core promoter elements
    Takashi Fukaya
    EMBL Conference Transcription and Chromatin, Online, Aug. 2020
  4. Synthetic biology in Japan
    Haruhiko Shiomi
    The BBSRC-JST UK-Japan Virtual Workshop in Synthetic Biology, Online, Oct. 2020
  5. CRISPR/Cas9 Mediated Genome Editing and Its Application for the Study of Fertilization in Mice
    Masahito Ikawa
    The University of Kansas Medical Center seminar, Online, Nov. 2020
  6. What do we need for the validation of disease model marmosets?
    Erika Sasaki
    Virtual Workshop on Validation of Animal Models and Tools for Biomedical Research, Online, Dec. 2020
     

 

  1. NELL2 is necessary for epididymal differentiation and male fertility
    淨住大慈
    令和元年 微生物病研究所 研究業績発表会 (大阪府吹田市) 2020.1.17
  2. Intergenerational epigenetic inheritance in mammals
    井上梓
    京都大学iPS研究所(CiRA) retreat 2019 (滋賀県大津市) 2020.2.14
  3. ピッグと並ぶもう1つの注目動物：マーモセット
    佐々木えりか
    自治医科大学先端医療技術開発センターシンポジウム2020 (栃木県下野市) 2020.2.10
  4. エピゲノム解析が明らかにする卵子・受精卵の特性
    石内崇士
    世界を先導するリプロダクションコアの形成研究会 (岡山県岡山市) 2020.2.27
  5. Identification of an epigenetic barrier between human embryonic and trophoblast stem cells
    岡江寛明
    第93回日本生化学会大会 (WEB)  2020.9.15
  6. Reprogramming somatic cell nuclei in Xenopus egg extracts
    新冨圭史
    第93回 日本生化学会大会 (WEB) 2020.9.15
  7. 分化全能性の消失に関わるインプリント遺伝子の同定
    岡江寛明
    第113回日本繁殖生物学会大会  (WEB) 2020.09.24
  8. マウス初期胚を用いた体細胞核の転写リプログラミング誘導
    宮本圭
    第113回日本繁殖生物学会大会  (WEB) 2020.09.24
  9. Topoisomerase IIa utilizes its C-terminal domain to work in crowded environments of mitotic chromosome assembly
    新冨圭史
    第43回 日本分子生物学会年会  (WEB) 2020.12.2

  1. 最優秀学術賞（大阪大学 微生物病研究所）
    淨住大慈 2020.01.17
  2. 総長賞（東北大学）
    全胞状奇胎由来幹細胞株の樹立と分子細胞学的検討
    高橋聡太​ ​​​​​​2020.03.25
  3. 教育貢献賞（東北大学大学院合同運営委員会）
    岡江寛明 2020.03.18
  4. 第52回 市村学術賞貢（公益財団法人 市村清新技術財団）
    齊藤 博英 2020.07.15
  5. 優秀発表賞（第113回日本繁殖生物学会大会）
    船屋 智史 2020.09.25
  6. 第15回生命医科学研究所ネットワーク国際シンポジウム若手口頭発表賞
    淨住大慈 2020.11.06
  7. 大阪大学賞（大阪大学）
    宮田治彦 2020.11.26
  8. 日本学術振興会賞（日本学術振興会）
    卵内初期化機構に関する研究
    宮本圭 2020.12.17

  1. 受精に必須の精子由来酵素を同定
    フジサンケイビジネスアイ　2019.02.20